Русский
Antioxidative activity and clinical efficiency of dietary supplements Nefradoz for treatment of recurrent calcium oxalate urolithiasis
DOI: https://dx.doi.org/10.18565/urology.2018.6.52-59
A.A. Kamalov, D.A. Okhobotov, E.V. Proskurnina, M.M. Sozarukova, A.N. Nizov
1 Research and Educational Center of Moscow State University by Lomonosov (Head of Department - Academician of the RAS, prof. A.A. Kamalov), Moscow, Russia; 2 Moscow State University by Lomonosov, Faculty of Fundamental Medicine, Department of Urology and Andrology (Head of Department - Academician of the RAS, prof. A.A. Kamalov), Moscow, Russia; 3 Moscow State University by Lomonosov, Faculty of Fundamental Medicine, Department of Medical Biophysics (Head of Department - Academician of the RAMS, Dr.Med.Sci., Yu.A. Vladimirov), Moscow, Russia; 4 National medical research center of cardiology, Institute of Clinical Cardiology named after A.L. Myasnikov, Moscow, Russia
Aim: to evaluate antioxidant properties of dietary supplements Nefradoz, as well as its role in the prevention of calcium oxalate stones.
Materials and methods. Our study consisted of two parts, clinical and experimental. In the clinical trial a total of 80 patients with recurrent calcium oxalate urolithiasis of various localization were included. All patients were divided into 2 groups. In treatment group (n=45) patients received Nefradoz 1 capsule 2 times a day. In control group (n=35) no treatment was prescribed. In all patients a biomarkers level (bikunin, osteopontin, nephrocalcin) in urine was determined by ELISA. In addition, Litos-test was performed. Follow-up duration was 6 months. In the experimental part of the study, the antioxidant properties of Nefradoz were evaluated using chemiluminescence method.
Results. The antioxidant activity of resveratrol, which is contained in 300 mg Nefradoz (1 capsule), was 23.66±1.18, which corresponds to the moderate activity. After 6 months of therapy a level of bikunin and osteopontin did not significantly changed, which indicates a minimal risk of stone recurrence. According to the Litos-test, the activity of stone formation in Nefradoz group significantly decreased to minimal values in 42 out 45 patients (p<0.05). In the control group an excretion of biomarkers was minimal, which significantly increased the risk of stone formation.
Conclusion. Dietary supplement NefraDoz can be used for prevention of calcium oxalate urolithiasis in case of increased stone formation according to the Litos-test. The antispasmodic, uroseptic and diuretic effects allow to maintain the effective level of biomarkers which is required for a recurrence-free course of urolithiasis compared to the control. The antioxidant activity, primarily determined by the resveratrol, can sufficiently compensate for oxidative stress and prevent the development of calcium oxalate stone. Further studies are needed to confirm our results.
Materials and methods. Our study consisted of two parts, clinical and experimental. In the clinical trial a total of 80 patients with recurrent calcium oxalate urolithiasis of various localization were included. All patients were divided into 2 groups. In treatment group (n=45) patients received Nefradoz 1 capsule 2 times a day. In control group (n=35) no treatment was prescribed. In all patients a biomarkers level (bikunin, osteopontin, nephrocalcin) in urine was determined by ELISA. In addition, Litos-test was performed. Follow-up duration was 6 months. In the experimental part of the study, the antioxidant properties of Nefradoz were evaluated using chemiluminescence method.
Results. The antioxidant activity of resveratrol, which is contained in 300 mg Nefradoz (1 capsule), was 23.66±1.18, which corresponds to the moderate activity. After 6 months of therapy a level of bikunin and osteopontin did not significantly changed, which indicates a minimal risk of stone recurrence. According to the Litos-test, the activity of stone formation in Nefradoz group significantly decreased to minimal values in 42 out 45 patients (p<0.05). In the control group an excretion of biomarkers was minimal, which significantly increased the risk of stone formation.
Conclusion. Dietary supplement NefraDoz can be used for prevention of calcium oxalate urolithiasis in case of increased stone formation according to the Litos-test. The antispasmodic, uroseptic and diuretic effects allow to maintain the effective level of biomarkers which is required for a recurrence-free course of urolithiasis compared to the control. The antioxidant activity, primarily determined by the resveratrol, can sufficiently compensate for oxidative stress and prevent the development of calcium oxalate stone. Further studies are needed to confirm our results.
Keywords: antioxidant activity, resveratrol, biomarkers of urinary stone disease, Nefradoz, bikunin, recurrent urolithiasis, osteopontin, oxidative stress
About the Autors
Corresponding author: Nizov A.N. – urologist at the Medical Research and Educational Center of Moscow State University by Lomonosov, researcher at the Department of Urology and Andrology, Faculty of Fundamental Medicine of Moscow State University by Lomonosov, Moscow, Russia; e-mail: nizovale@gmail.com
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