Русский
The association of GSTP1 polymorphisms with male idiopathic infertility
DOI: https://dx.doi.org/10.18565/urology.2019.2.50-54
N.A. Kurashova, E.V. Belyaeva, O.A. Ershova, K.D. Ievleva, B.G. Dashiyev, T.A. Bairova, L.I. Kolesnikova
1) Scientific Centre for Family Health and Human Reproduction Problems, Irkutsk, Russia; 2) Republican Perinatal Center of Ministry of Health of Republic of Buryatia, Ulan-Ude, Russia
Aim: To identify the association between Ile105Val, Ala114Val polymorphisms of gene GSTP1 (glutathione transferase pi1) and infertility in Russian men.
Materials and methods: A total of 160 infertile Russian men of reproductive age (mean age 30.2±3.6 years) were included in study group, while in control group there were 104 age-matched healthy fertile Russian male (mean age 31.3±5.4 years). Molecular and genetic studies of Ile105Val, Ala114Val polymorphisms of gene GSTP1 were performed using real-time polymerase chain reaction. Genomic DNA was extracted from the peripheral blood.
Results: A frequency of genotypes (p=0.024; df=2; p=0.024) and alleles (z=2,778; p=0,005) of Ile105Val locus was significantly different in the study and control group. In the study group, there was an increase in the frequency of the Ile/Ile genotype by 13% (χ²=3.995; df=1; p=0.046) and a decrease in the frequency of the Val/Val genotype by 6% (χ²=4.887; df=1; p=0.027). A rate of genotype Ile/Ile in infertile men with was 1.7 time higher than in control group (odds ratio (OR):1.73; 95% confidence interval (Cl): 1.04–2.87; p<0.05). There was not significant difference in frequency of genotypes and alleles of Ala114Val polymorphism of gene GSTP1 between the study and control groups.
Conclusions: The male infertility in Russia is associated with Ile105Val polymorphism and not associated with Ala114Val polymorphism of gene GSTP1. In infertile men wild-type genotype and major allele Ile105Val polymorphism of gene GSTP1 were significant more frequent compared to control group. A rate of genotype Ile/Ile in men with infertility was 1.7 time higher.
Materials and methods: A total of 160 infertile Russian men of reproductive age (mean age 30.2±3.6 years) were included in study group, while in control group there were 104 age-matched healthy fertile Russian male (mean age 31.3±5.4 years). Molecular and genetic studies of Ile105Val, Ala114Val polymorphisms of gene GSTP1 were performed using real-time polymerase chain reaction. Genomic DNA was extracted from the peripheral blood.
Results: A frequency of genotypes (p=0.024; df=2; p=0.024) and alleles (z=2,778; p=0,005) of Ile105Val locus was significantly different in the study and control group. In the study group, there was an increase in the frequency of the Ile/Ile genotype by 13% (χ²=3.995; df=1; p=0.046) and a decrease in the frequency of the Val/Val genotype by 6% (χ²=4.887; df=1; p=0.027). A rate of genotype Ile/Ile in infertile men with was 1.7 time higher than in control group (odds ratio (OR):1.73; 95% confidence interval (Cl): 1.04–2.87; p<0.05). There was not significant difference in frequency of genotypes and alleles of Ala114Val polymorphism of gene GSTP1 between the study and control groups.
Conclusions: The male infertility in Russia is associated with Ile105Val polymorphism and not associated with Ala114Val polymorphism of gene GSTP1. In infertile men wild-type genotype and major allele Ile105Val polymorphism of gene GSTP1 were significant more frequent compared to control group. A rate of genotype Ile/Ile in men with infertility was 1.7 time higher.
About the Autors
Corresponding author: E.V. Belyaeva – Ph.D. in Biology, Researcher at the Laboratory of Personalized Medicine, Scientific Centre for Family Health and Human Reproduction Problems, Irkutsk, Russia; e-mail: belyeva_irk@mail.ru
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