Русский
Comparative histological analysis of different materials used for urethroplasty in children with proximal forms of hypospadias: preputial skin vs buccal mucosa
DOI: https://dx.doi.org/10.18565/urology.2019.6.87-91
G.V. Kozyrev, D.T. Manasherova, G.A. Abdulkarimov, B.L. Kushnir, F.K. Abdullaev, V.V. Nikolaev, M.A. Gazimiev
1) Department of Uroandrology of the Russian Children’s Clinical Hospital, Moscow, Russia; 2) Faculty of Fundamental Medicine, Moscow State University, Moscow, Russia; 3) Department of Pathologic Anatomy of the Russian Children’s Clinical Hospital, Moscow, Russia; 4) I.M. Sechenov First Moscow State Medical University, Moscow, Russia
Aim: Buccal mucosa and preputial skin grafts are used for staged urethroplasty in proximal forms of hypospadias in children. Aim of our study was to carry out a comparative histological analysis of preputial skin and buccal mucosa.
Methods and materials: Histological analysis of urethral tissue samples from 10 patients with proximal forms of hypospadias was conducted. All patients were treated with staged Bracka’s technique using free grafts at the Russian Children’s Clinical Hospital from 2013 to 2016. Patients were divided into two groups. In Group I (n=5), preputial skin graft was used for urethroplasty, while in Group II (n=5) buccal mucosa was taken.
A histological study of both materials with comparison to a native urethra was performed.
Results: In Group I, the histological analysis showed keratinizing multilayered squamous epithelium, large number of dilated sweat and sebaceous glands with signs of inflammation, and hair follicles. In Group II, histological analysis revealed the presence of the typical structure for mucosal tissue, including multilayered flat non-keratinizing epithelium, as well as full absence of sweat, sebaceous glands, and hair follicles.
Conclusion: comparative histological analysis of neourethra has shown the absence of sweat and sebaceous glands, hair follicles, and areas of chronic inflammation in buccal mucosa vs preputial skin, showing that buccal mucosa is more similar to native urethra. Therefore, buccal mucosa is favored as the material for urethroplasty.
Methods and materials: Histological analysis of urethral tissue samples from 10 patients with proximal forms of hypospadias was conducted. All patients were treated with staged Bracka’s technique using free grafts at the Russian Children’s Clinical Hospital from 2013 to 2016. Patients were divided into two groups. In Group I (n=5), preputial skin graft was used for urethroplasty, while in Group II (n=5) buccal mucosa was taken.
A histological study of both materials with comparison to a native urethra was performed.
Results: In Group I, the histological analysis showed keratinizing multilayered squamous epithelium, large number of dilated sweat and sebaceous glands with signs of inflammation, and hair follicles. In Group II, histological analysis revealed the presence of the typical structure for mucosal tissue, including multilayered flat non-keratinizing epithelium, as well as full absence of sweat, sebaceous glands, and hair follicles.
Conclusion: comparative histological analysis of neourethra has shown the absence of sweat and sebaceous glands, hair follicles, and areas of chronic inflammation in buccal mucosa vs preputial skin, showing that buccal mucosa is more similar to native urethra. Therefore, buccal mucosa is favored as the material for urethroplasty.
About the Autors
Corresponding author: G.V. Kozyrev – Ph.D., urologist and andrologist, Department of Uroandrology of the Russian Children’s Clinical Hospital, Moscow, Russia; e-mail: kozgerman@mail.ru
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